AlphaLISA Prolactin Immunoassay Research kit, 5,000 assay points
Prolactin (PRL) is an endocrine factor primarily synthesized in the lactotrophs of the anterior pituitary but its production has also been proved in placenta, mammary epithelium and cancers, spleen, sweat gland, bone marrow, thymus, hypothalamus, skin fibroblasts and lymphocytes. PRL has several functions and autocrine and paracrine mechanisms have been shown. It stimulates growth, development and differentiation of breast epithelial cells, and promotes and maintains lactation during pregnancy and suckling. It also inhibits lipopropetin lipase activity in adipose tissue, shows angiogenic effects, and plays a role in the proliferation and differentiation of lymphocytes. The main form of human PRL is a 22 kDa globular protein (199 amino acids), but post-transcriptional and post-translational modifications such as alternative splicing, glycosylation, and proteolytic cleavage have been reported, leading to several forms from 14 to 23 kDa. PRL exerts its function through binding to the PRL Receptor (PRLR) presents in numerous tissues.
This AlphaLISA assay allows the detection of prolactin in buffer, cell culture media, serum and plasma in a highly sensitive, quantitative, reproducible and user-friendy mode.
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The protocol is easy, without wash steps and takes less than 3 hours to set up and run, thus allowing for easy adoption, decreased variability and results in half the time of a traditional ELISA.
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