Prolactin (PRL) is an endocrine factor primarily synthesized in the lactotrophs of the anterior pituitary but its production has also been proved in placenta, mammary epithelium and cancers, spleen, sweat gland, bone marrow, thymus, hypothalamus, skin fibroblasts and lymphocytes. PRL has several functions and autocrine and paracrine mechanisms have been shown. It stimulates growth, development and differentiation of breast epithelial cells, and promotes and maintains lactation during pregnancy and suckling. It also inhibits lipopropetin lipase activity in adipose tissue, shows angiogenic effects and plays a role in the proliferation and differentiation of lymphocytes. The main form of human PRL is a 22 kDa globular protein (199 amino acids), but post-transcriptional and post-translational modifications such as alternative splicing, glycosylation and proteolytic cleavage have been reported, leading to several forms from 14 to 23 kDa. PRL exerts its function through binding to the PRL Receptor (PRLR) present in numerous tissues.
This kit is designed for the quantitative determination of human prolactin in serum, buffered solution or cell culture medium using a homogeneous AlphaLISA assay (no wash steps).
Average Results
In AlphaLISA immunoassay buffer:
- Lower detection limit (LDL): 125 pg/mL
- Dynamic range: 125-300,000 pg/mL
Sensitivity can be increased by increasing the volume of analyte in the assay.
In analyte-depleted serum
- Lower detection limit (LDL): 367 pg/mL
- Dynamic range: 367-1,300,000 pg/mL
*For research use only.
The data was generated using a white OptiPlate-384 microplate and an EnVision-Alpha Reader 2102.
Each Kit Contains:
- AlphaLISA Acceptor beads coated with Anti-Analyte Antibody #1
- Streptavidin-coated Donor beads
- Biotinylated Anti-Analyte Antibody #2
- Lyophilized analyte
- AlphaLISA Immunoassay Buffer (10X).
Buffer and lyophilized analyte can be ordered separately.