AlphaLISA Epigenetic Cellular Detection Kits

Measure mark specific modulation of histone H3 in cell lysates

The AlphaLISA® Epigenetic Cellular Detection Kits enable rapid and direct detection of endogenous modification of epigenetic marks on histone H3. These no wash, all -in -one -well assays are optimized for simplicity and throughput.

Simple assay protocol amenable to automation, eliminating tedious ELISAs and Westerns
Suitable for endogenous and recombinant cell lines, providing the flexibility to work with relevant cell models
Ideal for HTS protocols with demonstrated Z’ factors greater than 0.6

Assay Overview The AlphaLISA assay is based on a 3 step assay: preparation, extraction and detection

Protocol legend: Homogeneous and highly specific AlphaLISA assays were developed to monitor the histone marks H3K4me2, H3K9ac, H3K27ac and H3K27me3 in high-throughput in cyto assays using an all-in-one well histone extraction protocol. These assays, which require only the addition of lysis buffer, histone extraction solution and detection reagents, were validated using adherent (HeLa and HEK-293) as well as suspension (Jurkat) cell lines.

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AlphaLISA Epigenetic Cellular Detection Kits

Products

AlphaLISA H3K27ac Cell Based Assay for Acetyl Modifications

AlphaLISA H3K27me3 Cell Based Assay for Methyl Modifications

AlphaLISA H3K4Me2 Cell Based Assay for Methyl Modifications

AlphaLISA H3K9ac Cell Based Assay for Acetyl Modifications

AlphaLISA® H3K27me2-1 Cell-based Assay Kit

AlphaLISA® H3K36me2 Cell-based Assay Kit

AlphaLISA® H3K4 (unmodified) Cell-based Assay Control Kit

AlphaLISA® H3K79me2 Cell-based Assay Kit

AlphaLISA® H3K9me2 Cell-based Assay Kit

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