Dear Dr. Vipoir:
I wish to design an experiment that shows co-localization of a single protein in a zebrafish cell. It is really important I capture a large single image of the zebrafish cell so I can see where my protein is. To do this I need to capture a single volume of 1024 x 1024 x 100. This application is extremely demanding and my system does not have enough virtual memory. What can I do? - Dr. D. Rerio
Dr Vipoir Says:-
Don’t worry Dr. D. Rerio we can all empathize with the stress and frustration caused by demanding applications. I am pleased to inform you that there is now a fully end-to-end 64-bit solution that could be the answer to your problems.
It is not possible to run the memory intensive iterative restoration on a single volume of 1024 x 1024 x 100 using Volocity on 32-bit Windows because, as you correctly put it, the system runs out of virtual memory. So what are your options when running Volocity on a 32-bit environment?
Crop the acquired data?
You could crop the image to 512 x 512 x 100 – but this loses spatial resolution or reduces the field of view.
Reduce the number of z slices to 1024 x 1024 x 40?
If these slices are still acquired through the same depth of sample, the axial resolution is dramatically reduced. If the same slice spacing is maintained less of the total sample depth is acquired which might exclude essential information.
Using either of these options on a 32-bit system is going to cause you to sacrifice what could be crucial information to your research.
Volocity acquisition on a 64-bit environment now provides you with a solution without sacrifice.
In choosing a fully 64-bit system you will have all the virtual memory you need and the reassurance that you can capture 100 % of your region of interest. Don’t let technology hold you back - take the right steps to ensuring you gain the data which could be essential to your science!
If you have an application that you think would benefit from 64-bit acquisition contact one of our imaging specialists now!