The PerkinElmer LS 55 Fluorescence spectrometer offers
flexibility, versatility, reliability and ease-of-use. Based on our
popular LS-50B, the LS 55 spectrometer includes a host of
auto-mated accessories and software to address a wide range of bio
research applications.
The LS 55 is built on PerkinElmer's heritage of sensitivity and
reliability. A wide range of automated accessories have also been
developed for the system making it the system ideal for
bioresearchers conducting:
- Microplate-based measurements
- Polarization
- Anisotropy assays
- Intact cell work
- Analysis of protein suspensions
Whether your application requires fluorescence, phosphorescence,
chemi - or bio-luminescence, the LS 55 is ready for the
challenge.
Optical performance you can count on
Our monochromator based LS 55 uses a high energy pulsed Xenon
source for excitation. For more than 20 years PerkinElmer has used
pulsed Xenon sources to minimize photo bleaching of samples and
provide a long-lived excitation source. The variable slit and
holographic gratings provide flexibility with very low stray
radiation. We''ve incorporated holographic gratings on both the
excitation and emission monochromators to further reduce stray
light and improve the system's already impressive performance. And
a newly designed reference signal system has been added to provide
users with much more control over signal dynamics. This is
particularly useful for samples with widely differing intra-sample
signal size. For example, the LS 55 signal system has reduced noise
by a factor of about 5 for intracellular ion analyses, and
increased data transfer rates by a factor of 10.
Powerful software controls your entire
analyses
Our FL WinLab™ software is designed for demanding
laboratories. It seamlessly combines PerkinElmer''s extensive
application specific knowledge and instrumentation control with the
ease-of-use provided by the Windows® operating environment.
Specific modes of instrument operation such as Scan, Time Drive,
and Ratio Data Collection can be easily accessed from the
Applications Menu (see Figure 1). Excitation and emission
monochromators can be independently or synchronously scanned, while
the Prescan mode is ideal for method development and locating of
peak excitation and emission maxima.
FL WinLab includes a validation protocol that automatically
checks the instrument performance to ensure that it is operating
within specification. With its unique set of accessories and
software applications, the LS 55 offers the most powerful and
flexible system for data collection and analysis.
Measurement Modes for the LS 55
- Fluorescence, phosphorescence and bio- and chemi-luminescence
measurement modes.
- Excitation, emission, constant wavelength synchronous, and
constant energy synchronous spectral scanning.
- 3D excitation/emission scans, 3D synchronous and kinetic
scans.
- Microplate measurements with fixed wavelength, wavelength
program or automated spectral data collection.
- TLC plate, electrophoresis gel or other flat sample types can
be analyzed with our Plate Reader accessory.
- Single and multiple wavelength kinetics
- Simultaneous kinetics for multiple samples
- Simple quantitation by curve fitting with a number of fit
algorithms
- Intracellular ion analyses
Accessories add sampling flexibility
The LS 55 includes a single cell thermostatted sample holder that
can accommodate 1 cm pathlength cells as well as semi-micro
cuvettes with or without stirring. Semi-micro cuvettes are
particularly useful where the sample is precious or volume is
limited such as cell cultures and DNA samples. The system also
includes an automated polarizer that consists of 2 filter wheels;
each wheel containing a horizontal and vertical polarizing element.
Polarizer positions are software controlled and can be manually set
or automatically controlled for polarization, anisotropy or
G-factor.
Accessories for the LS 55
Plate Reader accessory
The Plate Reader accessory is a sensitive and easy-to-use way to
measure fluorescence based assays in multi-well plates. Controlled
from the LS 55, it is able to use a wide range of UV and Visible
wavelengths ensuring the detection of virtually all fluorescent
dyes used in for bio assays.
Autopole system and software
The Autopole system and software automates the measurement of
polarization or anisotropy versus temperature. It includes a
temperature control system with temperature sensing built into the
cuvette.
Sipper accessory
A Sipper accessory is available for those laboratories that would
like to automate routine analysis and quantitation. It
automatically moves thesample from your sample vessel to the
measurement cuvette, and minimizes the need to handle and clean
quartz cuvettes.
Biokinetics accessory
The Biokinetics accessory consists of a single position stirred
cell holder with event marking and temperature sensing. It is
useful for all life science based work, but particularly useful for
polarization/anisotropy work, protein folding/unfolding, and DNA
melting.
Fast Filter accessory
The Fast Filter accessory is used for rapidly collecting data when
studying biochemical processes such as intracellular ion
monitoring. Pairs of optical filters specifically designed for the
indicator dyes are rotated rapidly in either the excitation or
emission beam allowing ratio measurements to be made every 40
millisecond. Two pairs of filters can be fitted on each wheel. The
ratio data or the individual intensities can be viewed in
real-time. For example, the Fast Filter allows for the simultaneous
measurement of both calcium and pH using a pair of FURA-2 and BCECF
filters.
4-position automatic Cell Changer
The 4-position automatic Cell Changer includes water
thermostatting and stirring for each sample position. This is
especially useful for simultaneous, multiple time-dependent
measurements from each of 4 cuvettes. Multiple samples can now be
multiplexed for analyses such as enzyme activities.
Front Surface accessory
The Front Surface accessory is ideal for analyzing films, papers,
powders and other flat samples. The sample may be placed in the
accessory directly or held in the powder holder that has a
synthetic fused silica window. Ultra small volumes or viscous
samples such as oils, opaque and turbid samples may be measured via
the holder or cuvette.
LC Flow Cell
The LC flow cell for high sensitivity liquid chromatography is a
good means of expanding the utility of the LS 55. Providing
laboratories with an alternative to a dedicated LC fluorescence
detector. The twin monochromators of the system enable accurate
wavelength selection for maximum selectivity and sensitivity.
Remote Fiber Optic accessory
The Remote Fiber Optic accessory allows you to take the
measurement at the sample rather than taking the sample to the
instrument. It is ideal for remote, non-destructive testing of
fluorescent papers and fabrics as well as the sampling of hazardous
materials such as radioactive sources.
AS-93plus Autosampler accessory
The high throughput AS-93plus Autosampler, when coupled to the LS
55, can measure up to 200 samples in a single rack. This provides a
high sensitivity alternative to microplate reading. The autosampler
has a built-in peristaltic pump that is controlled directly by our
FL WinLab software.