IVIS Spectrum Pre-clinical In Vivo Imaging System

Product number: IVISSPE

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The IVIS® Spectrum in vivo imaging system uses a novel patented optical imaging technology to facilitate non-invasive longitudinal monitoring of disease progression, cell trafficking and gene expression patterns in living animals. The IVIS Spectrum is a versatile and advanced in vivo imaging system. An optimized set of high efficiency filters and spectral un-mixing algorithms lets you take full advantage of bioluminescent and fluorescent reporters across the blue to near infrared wavelength region. It also offers single-view 3D tomography for both fluorescent and bioluminescent reporters that can be analyzed in an anatomical context using our Digital Mouse Atlas or registered with our multimodality module to other tomographic technologies such as MR, CT or PET.

For advanced fluorescence pre-clinical imaging, the IVIS Spectrum has the capability to use either trans-illumination (from the bottom) or epi-illumination (from the top) to illuminate in vivo fluorescent sources. 3D diffuse fluorescence tomography can be performed to determine source localization and concentration using the combination of structured light and trans illumination fluorescent images. The instrument is equipped with 10 narrow band excitation filters (30nm bandwidth) and 18 narrow band emission filters (20nm bandwidth) that assist in significantly reducing autofluorescence by the spectral scanning of filters and the use of spectral unmixing algorithms. In addition, the spectral unmixing tools allow the researcher to separate signals from multiple fluorescent reporters within the same animal. 

Get a quote on any of our systems or contact us with any questions you may have at invivoimaging@perkinelmer.com.

Resources
Video: IVIS Spectrum
Presentation: IVIS Imaging - Overview
In vivo Imaging Reagents
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Features/Benefits

  • High-sensitivity in vivo imaging of fluorescence and bioluminescence
  • High throughput (5 mice) with 23 cm field of view
  • High resolution (to 20 microns) with 3.9 cm field of view
  • Twenty eight high efficiency filters spanning 430 – 850 nm
  • Supports spectral unmixing applications
  • Ideal for distinguishing multiple bioluminescent and fluorescent reporters
  • Optical switch in the fluorescence illumination path allows reflection-mode or transmission-mode illumination
  • 3D diffuse tomographic reconstruction for both fluorescence and bioluminescence
  • Ability import and automatically co-register CT or MRI images yielding a functional and anatomical context for your scientific data.
  • NIST traceable absolute calibrations
  • Gas anesthesia inlet and outlet ports
  • Class I Laser Product

Application Literature

PerkinElmer recognizes the importance of proper training to ensure that our customers benefit fully from the capabilities of our instruments.Our Training Academy offers many training options; such as web based training, classroom or customized courses, to help you get the most out of your time in the lab.

Topics range from theory to techniques relating to specific methodology, optimization, and familiarization with instrumentation and related software. Courses are offered at various locations throughout the Americas, as well as Seer Green, England and Turku, Finland.

View our Customer Training Class Offerings (PDF)

Learn more about training.

Services & Support

With more than 1,300 service engineers, PerkinElmer provides service and support for instruments worldwide. In addition, our OneSource multivendor service engineers offer instrument repair, maintenance and regulatory compliance services for instruments from virtually any manufacturer.

Services and Support

Applications

  • Oncology Research (PDF)
  • Infectious Disease
  • Inflammation
  • Metabolic Diseases
  • Neurology
  • Gene Therapy
  • Stem cell Biology
  • Cardiovascular Disease
  • Immunology & Transplantation Biology
  • Toxicology (PDF)
  • Drug Metabolism Studies

Specifications

Power Requirements

20 Amps for 120 VAC or 10 Amps for 230 VAC

Dimensions

•Imaging Chamber Interior Size: 43 x 50 x 60 cm (W x D x H) •Imaging System Space Requirement: 203 x 163 x 214 cm (W x D x H)

Camera Sensor

Back-thinned, back-illuminated Grade 1 CCD

CCD

Size2.7 x 2.7 cm
Operating Temperature-90°C

Imaging Pixels

2048 x 2048

Quantum Efficiency

>85% 500 – 700 nm; >30% 400 – 900nm

Pixel Size

13.5 microns

Minimum Detectable Radiance

70 photons/s/sr/cm2

Field of View (FOV)

Minimum FOV3.9 x 3.9 cm
Maximum FOV23 x 23 cm

Minimum Image Pixel Resolution

20 microns

Read Noise

< 3 electrons for bin=1,2,4; < 5 electrons for bin=8,16

Dark Current (Typical)

<100 electrons/s/cm2

Filter Slots

Fluorescence Excitation12
Fluorescence Emission24

Fluorescence Filters

Excitation10
Emission18

Heated Chamber

Yes

Diffuse Tomography Software

Yes

Gas Anesthesia

Yes

Computer

2.8 GHz, 1 GB RAM, RW CD/DVD, 80 GB HD, 20” flat screen

Lens

f/1 – f/8, 1.5x, 2.5x, 5x, 8.7x magnifications

Stage Temperature

20 – 40°C

Transillumination Stage

Yes

Workbench

Yes