AlphaScreen® Protein A Acceptor beads, 5 mg
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AlphaScreen IgG detection kits are intended for the detection of
molecules recognized by a primary IgG using a secondary antibody or
protein A approach. Benefits of working with AlphaScreen
In comparison to TR-FRET assays, AlphaScreen allows the detection
of large molecules due to the proximity between the Donor and
Acceptor bead. The singlet oxygen can travel up to 200nm. In
addition, a large variety of affinities can be measured from very
low to very high. This is possible because the signal produced by
AlphaScreen results from the binding of dozens perhaps hundreds of
biopartners, rather than just one.Ease of assay optimization:
AlphaScreen beads contain the required dyes in order to get an
assay signal. This means you do not have to have custom fluorophore
labeling of your substrate, antibodies or molecules of interest.
The cascade reaction inside the Donor beads amplifies the signal
so very low concentrations of molecules can be detected.Very low backgrounds:
Due to the time resolved nature and the fact the emission
wavelength is lower than the excitation wavelength background
interference is extremely low.High signal to background ratio:
Because of the combination of strong signal and low background,
AlphaScreen S/B ratios tend to be outstanding, some assays reaching
S/B ratios of several hundreds.
For more information on working with AlphaScreen please refer to:
AlphaScreen Assay Principles
. Or contact
to consult with a PerkinElmer application scientist
who can support your assay development and help you choose the
right assay platform for your specific needs.
Technical Data can be found by entering the Product Number into our Certificate of Analysis Search Engine.
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