Fluoresence Tomography Systems

How important is tomography to your in vivo imaging results?

The FMT 2500 LX quantitative tomography system with PerkinElmer's suite of fluorescent agents provides the leading fluorescence tomographic imaging solution for quantification of deep tissue targets in vivo. Using one or multiple targeted, activatable and vascular agents and labels (for multiplexed results), a researcher can quantify a broad range of biologic targets, pathways and processes in vivo. In addition, the FMT is specifically developed for robust multi-modality imaging, allowing for the easy fusion of FMT with PET, MRI, CT and SPECT data sets. Whether you are imaging a particular biomarker, disease pathway or monitoring therapeutic efficacy, the FMT is easy to learn, fast to incorporate into a high-throughput workflow and quickly produces unmatched results.

Based on our proprietary FMT™ (Fluorescence Molecular Tomography) technology, the FMT 2500™ LX in vivo imaging system provides non-invasive, whole body, deep tissue quantitative in vivo imaging for preclinical applications.

  • Easily and efficiently obtain quantitative data in your animal models of disease
  • Measure and monitor multiple biological processes simultaneously
  • Generate 3D, information-rich results
  • Integrate FMT in vivo data with in vitro readouts from microscopy and cell-based imaging
  • Co-register data with CT, SPECT, PET or MR images
  • Use Reflectance mode for baseline comparison

Fluoresence Tomography combined with PerkinElmer's suite of fluorescent in vivo imaging agents and dyes enables scientists to:

  • Measure, monitor and quantify biological targets, processes and pathways in vivo
  • Deepen their understanding of disease mechanisms, disease progression and therapeutic responses
  • Use tomographic reconstructions to generate data sets from defined ROIs within the subject
  • Obtain functional and biological data to enhance data and decision-making in research and drug development projects
  • Monitor disease progression and therapeutic response in longitudinal studies
  • Integrate in vitro and in vivo data sets from profiling through translational research

FMT for Fluoresence Tomography

FMT for Fluoresence Tomography

FMT for Fluoresence Tomography: 3 Steps to Generate Fluoresence Tomographic Data in vivo

Quick to learn. Fast to image. Easy to quantify.

STEP 1: FMT Data Generation

  • Transillumination: FMT transillumination of animal using powerful 80 mW lasers
  • Creating Baseline Absorption Profiles: Baseline absorption profiles of each mouse's biological composition are measured by a laser-light raster scan. Paired absorption and fluorescence data maps generated from 10,000 - 100,000 source detector laser projections.
FMT laser-driven transillumination generates paired absorption and fluorescence data maps throughout the animal (image on right).		FMT Transillumination


The anesthetized mouse is comfortably placed in PerkinElmer's portable Animal Imaging Cassette. Imaging sessions are rapid (2-3 minutes per animal), animal handling is simple and the mouse remains stable and immobilized for consistent, repetitive imaging results (see image below).

Animal Cassette

STEP 2: FMT Normalization

  • Normalized fluorescence measurements generated by processing all paired absorption and fluorescence acquisition data.
  • FMT minimizes the distorting effects of in vivo tissue heterogeneity, taking into account surface boundaries, tissue spectral characteristics and wave-guiding effects to correct and normalize each fluorescent measurement.
  • Data normalized by FMT algorithmic models of photon transport in tissue.
FMT Normalization


Animal Cassette Workflow

Inserting the Animal Imaging Cassette into the FMT 2500.Easily position the anesthetized animal in the Imaging Cassette within the heated imaging chamber. Gas anesthesia is maintained.


STEP 3: FMT Reconstruction

  • Fluorescence quantified to the picomole at each point in the subject including deep tissue targets
  • Fluorescence measurements calculated throughout the user-selected regions of Interest (ROI)
  • Data provided in universal formats for analysis (including DICOM), database building, and decision-making
  • Easily create animated movies including dynamic presentation of quantification data for presentation
  • Data and images easily exported for multi-modality image fusion
  • Quantification is performed on all animals at any depth with equal accuracy and without the need for an artificial image atlas for referential quantification.


FMT Reconstruction

FMT Reconstruction

Easy Experiment Set-UpQuick Tomographic ScanQuantitative Analysis


Select either the FMT 1500™ or the FMT 2500™ LX for your unique quantitative in vivo imaging needs.

Whatever your needs, PerkinElmer has a quantitative FMT platform to provide deeper insights to your biological questions. Select the FMT 1500 for quantitative imaging in individual laboratories, in smaller facilities or for single applications. The 4-channel FMT 2500 LX is available for laboratories requiring a broader range of applications and extensive multi-modality capabilities.

FMT 1500™ Fluoresence Tomography in vivo Imaging System
FMT 1500		FMT 2500™ LX Fluoresence Tomography in vivo Imaging System
FMT 2500 LX

Whether you are imaging a particular biomarker, disease pathway or monitoring therapeutic efficacy, the FMT is easy to learn, fast to incorporate into a high-throughput workflow and quickly produces unmatched results.

Cardiovascular
Cardiovascular
Pulmonary
Pulmonary
Co-registered FMT-MRI imaging data of proteolytic activities validating splenic deployment of monocytes to the heart followingmyocardial infarction.*

*Image courtesy of Swirski et al, Science, 31 July 2009 Vol 325, pp. 612


Eosinophil cathepsin activity quantified with FMT and ProSense in an ovalbumin-induced asthma model (right); not visible with planar fluorescence (left).


CNS
CNS
Oncology
Oncology
FMT tomographic imaging in brain for EAE disease severity. Quantification of AngioSense.


FMT quantitative imaging of breast cancer metastasis to the lung, using ProSense (right); not visible with planar fluorescence (left).


Bone and Skeletal DiseaseBone and Skeletal DiseaseInflammation
Inflammation
FMT quantitative imaging of arthritis using ProSense to quantify arthritic inflammation in vivo in CAIA mice.


FMT quantification of acute edema and cellular inflammatory response. Quantification of AngioSense.


Infectious Disease

Infectious Disease
FMT quantitative imaging of Schistosomiasis using ProSense showing infection of liver and lower gastrointestinal tract.