AlphaLISA® Human VEGF Research Immunoassay Kit
FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES.
Human Vascular Endothelial Growth Factor (VEGF-A or VEGF)is a homodimeric 34-42 kDa heparin-binding glycoprotein specific for endothelial cells. Currently, at least eight variants of human VEGF generated by alternative splicing of the same gene have been identified. The various isoforms have different heparin- and neuropilin (-1 or -2)-binding properties as well as solubility characteristics reflecting the different functional properties of the VEGF forms.
VEGF is believed to play important roles in inflammation and during normal and pathological angiogenesis, a process that is associated with wound healing, embryonic development, and growth and metastasis of solid tumors. Elevated levels of VEGF have been observed in synovial fluids of rheumatoid arthritis patients and cancer patients' sera. The AlphaLISA VEGF kit is designed for the quantitative determination of human VEGF-A in serum, plasma, buffered solution or cell culture medium. It can detect VEGF165 (isoform d), VEGF162, VEGF145 (isoform e) and VEGF121 (isoform f).
In AlphaLISA Immunoassay Buffer:
- Lower Detection Limit (LDL): 2.2 pg/mL
- Dynamic Range: 2.2-100,000 pg/mL
Each Kit Contains:
- AlphaLISA Acceptor beads coated with Anti-Analyte Antibody #1
- Streptavidin-coated Donor beads
- Biotinylated Anti-Analyte Antibody #2
- Lyophilized analyte
- AlphaLISA Immunoassay Buffer (10X).
Buffer and lyophilized analyte can be ordered separately