AlphaLISA NS0-P Immunoassay Research kit

NSO murine hybridoma cells are commonly utilized as an expression system for the production of human recombinant proteins and monoclonal antibodies at industrial scale. As therapeutical agents for humans, they must be subsequently purified to remove undesired contaminants that could elicit toxic or immunological reactions. Therefore, there is a current need to quantitate and trace these unwanted host cell proteins in protein preparations with excellent sensitivity and robustness, while maintaining high throughput capabilities.

This kit is designed for the quantitative determination of NSO host cell proteins in buffered solution or cell culture medium using a homogeneous AlphaLISA assay (no wash steps).

Average Results

In AlphaLISA HiBlock buffer:

  • Lower detection limit (LDL): 1.6 ng/mL
  • Dynamic range: 1.6-1,000 ng/mL

Sensitivity can be increased by increasing the volume of analyte in the assay.
For research use only.
The data was generated using a white OptiPlate-384 microplate and an EnVision-Alpha Reader 2102.

alphalisa NSO_P.jpg

alphalisa NSO_P specificity.jpg

Each Kit Contains:

  • AlphaLISA Acceptor beads coated with Anti-Analyte Antibody #1
  • Streptavidin-coated Donor beads
  • Biotinylated Anti-Analyte Antibody #2
  • Lyophilized analyte
  • AlphaLISA HiBlock buffer (10X).

Buffer and lyophilized analyte can be ordered separately.