AlphaLISA technology allows the detection of molecules of interest in a no-wash, highly sensitive, quantitative assay. In an AlphaLISA assay, a biotinylated anti-analyte antibody binds to the Streptavidin-coated Donor beads while another anti-analyte antibody is conjugated to AlphaLISA Acceptor beads. In the presence of the analyte, the beads come into close proximity. The excitation of the Donor beads causes the release of singlet oxygen molecules that triggers a cascade of energy transfer in the Acceptor beads, resulting in a sharp peak of light emission at 615 nm.
NSO murine hybridoma cells are commonly utilized as an expression system for the production of human recombinant proteins and monoclonal antibodies at industrial scale. As therapeutical agents for humans, they must be subsequently purified to remove undesired contaminants that could elicit toxic or immunological reactions. Therefore, there is a current need to quantitate and trace these unwanted host cell proteins in protein preparations with excellent sensitivity and robustness, while maintaining high throughput capabilities.
This kit is designed for the quantitative determination of NSO host cell proteins in buffered solution or cell culture medium using a homogeneous AlphaLISA assay (no wash steps).
In AlphaLISA HiBlock buffer:
- Lower detection limit (LDL): 1.6 ng/mL
- Dynamic range: 1.6-1,000 ng/mL
Sensitivity can be increased by increasing the volume of analyte in the assay.
For research use only.
The data was generated using a white OptiPlate-384 microplate and an EnVision-Alpha Reader 2102.
Each Kit Contains:
- AlphaLISA Acceptor beads coated with Anti-Analyte Antibody #1
- Streptavidin-coated Donor beads
- Biotinylated Anti-Analyte Antibody #2
- Lyophilized analyte
- AlphaLISA HiBlock buffer (10X).
Buffer and lyophilized analyte can be ordered separately.