Interleukin 2 (IL2), formerly known as T-cell Growth Factor (TCGF), is a 15 kDa immunoregulatory lymphokine known to be produced by lectin- or antigen-activated T cells and capable of inducing the thymic expansion of recently activated antigen-specific T lymphocytes. However, since T-cell immunity could be elicited to various agents in the absence of IL2 in vivo, it is currently thought that the main nonredundant activity of this cytokine is the induction of the suppressor function of CD4+CD25+ regulatory T cells (Treg) in peripheral lymph nodes to ensure suppression of autoreactive T cells that escape negative selection. Moreover, recent findings suggest that IL2- mediated regulation of Treg cells is important in the prevention of type 1 diabetes and autoimmune disease.
This kit is designed for the quantitative determination of human IL2 in serum, buffered solution or cell culture medium using a homogeneous AlphaLISA assay (no wash steps).
Average Results
In AlphaLISA immunoassay buffer:
- Lower detection limit (LDL): 1.8 pg/mL
- Dynamic range: 1.8-100,000 pg/mL
Sensitivity can be increased by increasing the volume of analyte in the assay.
In analyte-depleted serum:
- Lower detection limit (LDL): 22.2 pg/mL
- Dynamic range: 22.2-100,000 pg/mL
For research use only.
The data was generated using a white OptiPlate-384 microplate and an EnVision-Alpha Reader 2102.
Each Kit Contains:
- AlphaLISA Acceptor beads coated with Anti-Analyte Antibody #1
- Streptavidin-coated Donor beads
- Biotinylated Anti-Analyte Antibody #2
- Lyophilized analyte
- AlphaLISA Immunoassay Buffer (10X)
Buffer and lyophilized analyte can be ordered separately.