DELFIA® TRF Assays

DELFIA (Dissociation-Enhanced Lanthanide Fluorescent Immunoassay) is a robust, high-performance immunodetection platform that provides a combination of benefits that make it the superior alternative to conventional ELISA. DELFIA utilizes the unique chemical properties of lanthanide chelates in concert with time-resolved fluorescence (TRF) detection to create an assay that offers high sensitivity, wide dynamic range, superior stability, and excellent flexibility. Given the robust nature of DELFIA technology and the advantages it holds over traditional ELISA, it is not surprising that DELFIA has found widespread use in research, drug discovery, and clinical environments.

delfiaassayprinciple.jpg

Features/Benefits: High Sensitivity

  • Femtogram-level detection - detect low concentration targets and rare biological events that would be missed by less sensitive methods. Ideal for complex sample matrices
  • Superior sensitivity means less sample and less antibody is required - translating into significant cost savings
  • Use DELFIA with PerkinElmer’s VICTOR™ X Multilabel Plate Reader with filter-based time-resolved fluorescence for maximum sensitivity
Wide Dynamic Range
  • DELFIA offers a 4-5 log dynamic range - as much as 2-fold greater than traditional ELISA
  • Sample preparation and assay repeats are minimized, resulting in reduced assay times and cost savings
Superior Stability
  • DELFIA technology is based on lanthanide chemistry, eliminating concerns about enzyme quality, degradation or sample contamination.
  • Lanthanide chelates are extremely stable, allowing for delayed signal detection and batch processing experimental workflows.
Excellent Flexibility
  • Easily converted to 384-well format
  • Multiplex capabilities - multiplex up to 3 analytes per well
  • Wide range of applications, including:
    • Basic Immunodetection
    • Receptor-Ligand Binding
    • Enzyme Assays
    • Cell Cytotoxicity and Proliferation Assays
    • Protein-Protein and Protein-DNA Interaction Studies
    • Biodistribution Studies

Quick Links


Products

DELFIA Detection Reagents

DELFIA is a proven, robust immuno-detection platform based on time-resolved fluorescence (TRF) detection....  View more

DELFIA Labeling Services

The DELFIA labeling service provides custom labeling of biomolecules with the lanthanide chelates Europium...  View more

DELFIA Microplates

DELFIA® assays can be performed using either coated microplates or filter plates. The main critical ...  View more

DELFIA® Cytotoxicity and Proliferation Assays

Cell proliferation is an important parameter when studying live cell function, especially when the effect...  View more

DELFIA® Kinase Assays

Protein phosphorylation is a basic mechanism for the modification of protein function in eukaryotic ...  View more

DELFIA® Labeling Reagents

N1 chelates The most commonly used chelate is the N1 chelate. It allows easy labeling and purification,...  View more