LANCE® TR-FRET Assays

Ideal for HTS and ultra HTS, LANCE biochemical TR-FRET assays are sensitive, homogeneous and easy to use.

A history of innovation

The first company to commercialize europium-based TR-FRET, PerkinElmer is actively evolving TR-FRET’s performance as well as its applications. We have over 30 years’ experience in defining, synthesizing and optimizing Europium chelate and chelate-based bioanalytical assays.

A clearer signal

TR-FRET assays combine the advantages of time resolution (TR) with fluorescence resonance energy transfer (FRET) principles, using donor and acceptor fluorophore labels. When the two fluorophore labels are brought together, energy can be transferred from donor to acceptor. As a consequence, excitation of the donor fluorophore will result in emission from the acceptor fluorophore when the fluorophores are in close proximity (~ 9 nm or less). The level of light emitted from the acceptor fluorophore is proportional to the degree of donor-acceptor complex formation.
WHICH LANCE TR-FRET ASSAY IS RIGHT FOR YOU?
Are you trying to measure biochemical kinase activity?LANCE® Kinase Reagents
Studying Gi or Gs coupled GPCRs?LANCE® and LANCE Ultra
cAMP Kits for GPCR Assays
Measuring protein:protein interactions
or other binding / enzymatic assays?
LANCE® Toolbox Reagents
Working with full length substrates,
cellular kinases, or complex samples?
Try our Alpha Technology
Are you trying to detect methylation
or acetylation states of peptide histone proteins?
LANCE® Epigenetics Reagents

A choice of platforms

PerkinElmer offers two TR-FRET platforms: the original LANCE (Lanthanide Chelate Excite) and the next-generation LANCE Ultra. Both use europium (Eu) chelate donor dyes, but have different acceptor fluorophores:

ULight™ acceptor dye (LANCE Ultra): Small dye (<800 Da), ideal for direct coupling/direct labeling of molecules of any size (including small molecules and peptides), reduced steric hindrance to labeled molecule, excellent for enzymatic assays using directly-labeled substrates

SureLight® APC acceptor dye (LANCE): Larger dye (>100,000 Da) derived from a light-harvesting protein, can act as large antenna, ideal for indirect assays that span wider distances, excellent for protein-protein interactions

apc
LANCE TR-FRET assay using LANCE Europium and SureLight® APC

U Light
LANCE TR-FRET assay using LANCE Europium and ULight dye.

A wide variety of applications has been studied using LANCE TR-FRET technology, including kinase assays, GPCR activation, protein-protein binding, protein-nucleic acid binding, protein-small molecule binding, receptor-ligand binding, nuclear receptors, receptor dimerization, post-translational modifications, biomarker assays, proteases, and more.

For a total solution, we offer a range of automation and detection systems, such as the VICTOR™ Multilabel Plate reader, optimized for use with our assays and reagents.


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Products

LANCE® and LANCE Ultra cAMP Kits for GPCR Assays

LANCE®Ultra cAMP and LANCE® cAMP Assays: Robust and ready for uHTS! Measure cyclic AMP produced by ...  View more

LANCE® Toolbox Reagents

The versatility of LANCE TR-FRET technology allows the assembly of donor and acceptor dye pairs for ...  View more

LANCE® TR-FRET Epigenetics Tool Box

Consistent, validated solutions for your most demanding epigenetics applications. - Proven, cost-efficient...  View more

LANCE® TR-FRET Kinase Reagents

Over 310 serine, threonine and tyrosine kinases have been validated for performance and reactivity with...  View more

OnPoint Custom Labeling Services

Your Reagents Made to OrderWhatever the assay you have in mind, you need the right technologies for ...  View more