Radioligand binding assays are an important tool in many disciplines in biological sciences, both in academic and industrial laboratories. It evaluates the interaction of a molecule (ligand) specifically binding to a protein (receptor).
- The most frequently used assay is the membrane filtration receptor assay
- Filtration assays are used for speed and convenience
- Applicable to most known neurotransmitter and hormone receptor systems
- 3H radioligands can be left chemically unaltered, and thus, biologically indistinguishable from the unlabeled compound, and they have a longer half-life (12 years vs. 60 days)
- Iodinated radioligands have a higher specific activity, which makes them particularly useful if the density of receptors is low
PerkinElmer offers a comprehensive collection of validated fresh cell lines, frozen cells, membrane ...
FlashPlate® is a white polystyrene microplate, designed for
high-volume, homogeneous radiometric assays,...
Scintillation proximity assay (SPA) is a homogeneous and versatile
technology for the rapid and sensitive...