DELFIA Dissociation-enhancement Solutions
In most DELFIA assays, the labeled biomolecule as such is
practically non-fluorescent. However, after the binding reaction is
complete fluorescence is developed by the addition of either
Enhancement Solution or DELFIA Inducer. Both of these are suitable
for the detection of both europium and samarium. DELFIA Inducer is
used for all DELFIA chelates of these lanthanides, while
Enhancement Solution is recommended for N1 chelates only. The speed
of the dissociation enhancement process will depend on the original
chelate used, the choice of DELFIA Enhancement Solution or DELFIA
Inducer, and whether or not shaking is used. 50 mL of either DELFIA
Enhancement Solution or DELFIA Inducer is sufficient for 250 wells
of a standard 96-well microplate.
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